Direct regulation of the cardiac ryanodine receptor (RyR2) by O-GlcNAcylation.

ABSTRACT:

Background

O-GlcNAcylation is the enzymatic addition of a sugar, O-linked β-N-Acetylglucosamine, to the serine and threonine residues of proteins, and is abundant in diabetic conditions. We have previously shown that O-GlcNAcylation can trigger arrhythmias by indirectly increasing pathological Ca²⁺ leak through the cardiac ryanodine receptor (RyR2) via Ca²⁺/calmodulin-dependent kinase II (CaMKII). However, RyR2 is well known to be directly regulated by other forms of serine and threonine modification, therefore, this study aimed to determine whether RyR2 is directly modified by O-GlcNAcylation and if this also alters the function of RyR2 and Ca²⁺ leak.

Methods

O-GlcNAcylation of RyR2 in diabetic human and animal hearts was determined using we

SUBMITTER: Okolo CA 

PROVIDER: S-EPMC10576323 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature