Cont-ID: detection of sample cross-contamination in viral metagenomic data.

ABSTRACT:

Background

High-throughput sequencing (HTS) technologies completed by the bioinformatic analysis of the generated data are becoming an important detection technique for virus diagnostics. They have the potential to replace or complement the current PCR-based methods thanks to their improved inclusivity and analytical sensitivity, as well as their overall good repeatability and reproducibility. Cross-contamination is a well-known phenomenon in molecular diagnostics and corresponds to the exchange of genetic material between samples. Cross-contamination management was a key drawback during the development of PCR-based detection and is now adequately monitored in routine diagnostics. HTS technologies are facing similar difficulties due to their very high analytical sensitivity. As a s

SUBMITTER: Rollin J 

PROVIDER: S-EPMC10576407 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature