Biophysical, Molecular and Proteomic Profiling of Human Retinal Organoid-Derived Exosomes.

ABSTRACT:

Purpose

There is a growing interest in extracellular vesicles (EVs) for ocular applications as therapeutics, biomarkers, and drug delivery vehicles. EVs secreted from mesenchymal stem cells (MSCs) have shown to provide therapeutic benefits in ocular conditions. However, very little is known about the properties of bioreactor cultured-3D human retinal organoids secreted EVs. This study provides a comprehensive morphological, nanomechanical, molecular, and proteomic characterization of retinal organoid EVs and compares it with human umbilical cord (hUC) MSCs.

Methods

The morphology and nanomechanical properties of retinal organoid EVs were assessed using Nanoparticle tracking analysis (NTA) and Atomic force microscopy (AFM). Gene expression analysis of exosome biogenesis of ea

SUBMITTER: Arthur P 

PROVIDER: S-EPMC10576571 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature