Miniature spatial transcriptomics for studying parasite-endosymbiont relationships at the micro scale.

ABSTRACT: Several important human infectious diseases are caused by microscale-sized parasitic nematodes like filarial worms. Filarial worms have their own spatial tissue organization; to uncover this tissue structure, we need methods that can spatially resolve these miniature specimens. Most filarial worms evolved a mutualistic association with endosymbiotic bacteria Wolbachia. However, the mechanisms underlying the dependency of filarial worms on the fitness of these bacteria remain unknown. As Wolbachia is essential for the development, reproduction, and survival of filarial worms, we spatially explored how Wolbachia interacts with the worm's reproductive system by performing a spatial characterization using Spatial Transcriptomics (ST) across a posterior region containing reproductive tissue and

SUBMITTER: Sounart H 

PROVIDER: S-EPMC10576761 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature