Reverse pH-dependent fluorescence protein visualizes pattern of interfacial proton dynamics during hydrogen evolution reaction.

ABSTRACT: Reverse pH-dependent fluorescent protein, including dKeima, is a type of fluorescent protein in which the chromophore protonation state depends inversely on external pH. The dependence is maintained even when immobilized at the metal-solution interface. But, interestingly, its responses to the hydrogen evolution reaction (HER) at the interface are not reversed: HER rises the pH of the solution around the cathode, but, highly active HER induces chromophore deprotonation regardless of the reverse pH dependence, reflecting an interface-specific deprotonation effect by HER. Here, we exploit this phenomenon to perform scanning-less, real-time visualization of interfacial proton dynamics during HER at a wide field of view. By using dKeima, the HER-driven deprotonation effect was well discriminat

SUBMITTER: Farha TD 

PROVIDER: S-EPMC10577132 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature