CH02 peptide promotes ex vivo expansion of umbilical cord blood-derived CD34 ⁺ hematopoietic stem/progenitor cells.

ABSTRACT: Umbilical cord blood (UCB) is an advantageous source for hematopoietic stem/progenitor cell (HSPC) transplantation, yet the current strategies for large-scale and cost-effective UCB-HSPC preparation are still unavailable. To overcome these obstacles, we systematically evaluate the feasibility of our newly identified CH02 peptide for ex vivo expansion of CD34 ⁺ UCB-HSPCs. We herein report that the CH02 peptide is specifically enriched in HSPC proliferation via activating the FLT3 signaling. Notably, the CH02-based cocktails are adequate for boosting 12-fold ex vivo expansion of UCB-HSPCs. Meanwhile, CH02-preconditioned UCB-HSPCs manifest preferable efficacy upon wound healing in diabetic mice via bidirectional orchestration of proinflammatory and anti-inflammatory f

SUBMITTER: Yang Y 

PROVIDER: S-EPMC10577473 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature