Fingerstick blood assay maps real-world NAD⁺ disparity across gender and age.

ABSTRACT: Nicotinamide adenine dinucleotide (NAD⁺ ) level has been associated with various age-related diseases and its pharmacological modulation emerges as a potential approach for aging intervention. But human NAD⁺ landscape exhibits large heterogeneity. The lack of rapid, low-cost assays limits the establishment of whole-blood NAD⁺ baseline and the development of personalized therapies, especially for those with poor responses towards conventional NAD⁺ supplementations. Here, we developed an automated NAD⁺ analyzer for the rapid measurement of NAD⁺ with 5 μL of capillary blood using recombinant bioluminescent sensor protein and automated optical reader. The minimal invasiveness of the assay allowed a frequent and decentralized map

SUBMITTER: Wang P 

PROVIDER: S-EPMC10577551 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature