Investigation of effects of orthokeratology and povidone iodine disinfecting solution on the conjunctival microbiome using MALDI-TOF mass spectrometry.

ABSTRACT:

Purpose

To determine organisms present in the conjunctiva of children before and after orthokeratology lens wear, using MALDI-TOF mass spectrometry.

Methods

Conjunctival samples were collected from children aged 8-12 years (inclusive) at baseline and on three occasions over the first six months of orthokeratology treatment. All lenses were disinfected using the povidone iodine-based solution every day after use. Specimens were cultured and all isolated colonies were identified using MALDI-TOF mass spectrometry. Numbers of organisms and diversity were compared over the study period and the presence of any ocular pathogens noted and participants informed, where appropriate, to enhance their compliance with lens care routine.

Results

Organisms isolated from 76 children w

SUBMITTER: Boost MV 

PROVIDER: S-EPMC10577863 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature