Vitamin C activates young LINE-1 elements in mouse embryonic stem cells via H3K9me3 demethylation.

ABSTRACT:

Background

Vitamin C (vitC) enhances the activity of 2-oxoglutarate-dependent dioxygenases, including TET enzymes, which catalyse DNA demethylation, and Jumonji-domain histone demethylases. The epigenetic remodelling promoted by vitC improves the efficiency of induced pluripotent stem cell derivation, and is required to attain a ground-state of pluripotency in embryonic stem cells (ESCs) that closely mimics the inner cell mass of the early blastocyst. However, genome-wide DNA and histone demethylation can lead to upregulation of transposable elements (TEs), and it is not known how vitC addition in culture media affects TE expression in pluripotent stem cells.

Results

Here we show that vitC increases the expression of several TE families, including evolutionarily young LINE-1

SUBMITTER: Cheng KCL 

PROVIDER: S-EPMC10578016 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature