Expression of the gene encoding blood coagulation factor VIII without domain B in E. coli bacterial expression system.

ABSTRACT: In this article, we have demonstrated the feasibility of generating an active form of recombinant blood coagulation factor VIII using an E. coli bacterial expression system as a potential treatment for hemophilia type A. Factor VIII (FVIII), an essential blood coagulation protein, is a key component of the fluid phase blood coagulation system. So far, all available recombinant FVIII formulations have been produced using eukaryotic expression systems. Mammalian cells can produce catalytically active proteins with all the necessary posttranslational modifications. However, cultivating such cells is time-consuming and highly expensive, and the amount of the obtained product is usually low. In contrast to eukaryotic cells, bacterial culture is inexpensive and allows the acquisition of l

SUBMITTER: Mazurkiewicz-Pisarek A 

PROVIDER: S-EPMC10578111 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature