Investigating protein-membrane interactions using native reverse micelles constructed from naturally sourced lipids.

ABSTRACT: Advancing the study of membrane associated proteins and their interactions is dependent on accurate membrane models. While a variety of membrane models for high-resolution membrane protein study exist, most do not reflect the diversity of lipids found within biological membranes. In this work, we have developed native reverse micelles (nRMs) formulated with lipids from multiple eukaryotic sources, which encapsulate proteins and enable them to interact as they would with a biological membrane. Diverse formulations of nRMs using soy lecithin, porcine brain lipids, or bovine heart lipids combined with n-dodecylphosphocholine were developed and characterized by dynamic light scattering and ³¹ P-NMR. To optimize protein encapsulation, ubiquitin was used as a standard and protein NMR

SUBMITTER: Walters SH 

PROVIDER: S-EPMC10578115 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature