Theoretical study of ArcB and its dimerization, interaction with anaerobic metabolites, and activation of ArcA.

ABSTRACT: The complex metabolism of Escherichia coli has been extensively studied, including its response to oxygen availability. The ArcA/B two-component system (TCS) is the key regulator for the transition between these two environmental conditions and has been thoroughly characterized using genetic and biochemical approaches. Still, to date, limited structural data is available. The breakthrough provided by AlphaFold2 in 2021 has brought a reliable tool to the scientific community for assessing the structural features of complex proteins. In this report, we analyzed the structural aspects of the ArcA/B TCS using AlphaFold2 models. The models are consistent with the experimentally determined structures of ArcB kinase. The predicted structure of the dimeric form of ArcB is consistent with th

SUBMITTER: Padilla-Vaca F 

PROVIDER: S-EPMC10578306 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature