Development and clinical validation of a microfluidic-based platform for CTC enrichment and downstream molecular analysis.

ABSTRACT:

Background

Although many CTC isolation and detection methods can provide information on cancer cell counts, downstream gene and protein analysis remain incomplete. Therefore, it is crucial to develop a technology that can provide comprehensive information on both the number and profile of CTC.

Methods

In this study, we developed a novel microfluidics-based CTC separation and enrichment platform that provided detailed information about CTC.

Results

This platform exhibits exceptional functionality, achieving high rates of CTC recovery (87.1%) and purification (∼4 log depletion of WBCs), as well as accurate detection (95.10%), providing intact and viable CTCs for downstream analysis. This platform enables successful separation and enrichment of CTCs from a 4 mL whole-blo

SUBMITTER: Cai S 

PROVIDER: S-EPMC10578963 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature