Integrative analysis of cellular autophagy-related genes in steroid-induced osteonecrosis of the femoral head.

ABSTRACT:

Objective

This study aimed to identify and evaluate genes associated with cellular autophagy in steroid hormonal femoral head necrosis.

Methods

Autophagy-related differentially expressed genes (ARDEGs) in steroid-induced osteonecrosis of the femoral head (SONFH) were identified by obtaining the intersection of differentially expressed genes (DEGs) and autophagy-related genes in a SONFH Gene Expression Omnibus dataset. The ARDEGs were screened, and correlations between gene expression and immune cell infiltration were evaluated. Finally, the validation of hub genes was undertaken through quantitative real-time-PCR.

Results

A comparison of peripheral blood samples from patients with and without SONFH revealed 189 DEGs. Gene Ontology, Kyoto Encyclopedia of Genes and Geno

SUBMITTER: Wang Y 

PROVIDER: S-EPMC10579027 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature