Activation of MG53 Enhances Cell Survival and Engraftment of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Injured Hearts.

ABSTRACT:

Background and objective

Our previous studies demonstrated that MG53 protein can protect the myocardium, but its use as a therapeutic is challenging due to its short half-life in blood circulation. This study aimed to investigate the cardioprotective role of MG53 on human induced pluripotent stem cell-derived cardiomyocytes (HiPSC-CMs) in the context of myocardial ischemia/reperfusion (I/R).

Methods

In vitro: HiPSC-CMs were transfected with adenoviral MG53 (HiPSC-CMs^MG53), in which the expression of MG53 can be controlled by doxycycline (Dox), and the cells were then exposed to H₂O₂ to mimic ischemia/reperfusion injury. In vivo: HiPSC-CMs^MG53 were transplanted into the peri-infarct region in NSG™ mice after I/R. After surgery, mice

SUBMITTER: Park KH 

PROVIDER: S-EPMC10579131 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature