Cartilage-specific <i>Sirt6</i> deficiency represses IGF-1 and enhances osteoarthritis severity in mice.

ABSTRACT:

Objectives

Prior studies noted that chondrocyte SIRT6 activity is repressed in older chondrocytes rendering cells susceptible to catabolic signalling events implicated in osteoarthritis (OA). This study aimed to define the effect of Sirt6 deficiency on the development of post-traumatic and age-associated OA in mice.

Methods

Male cartilage-specific Sirt6-deficient mice and Sirt6 intact controls underwent destabilisation of the medial meniscus (DMM) or sham surgery at 16 weeks of age and OA severity was analysed at 6 and 10 weeks postsurgery. Age-associated OA was assessed in mice aged 12 and 18 months of age. OA severity was analysed by micro-CT, histomorphometry and scoring of articular cartilage structure, toluidine blue staining and osteophyte formatio

SUBMITTER: Collins JA 

PROVIDER: S-EPMC10579179 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature