Core clock gene BMAL1 and RNA-binding protein MEX3A collaboratively regulate Lgr5 expression in intestinal crypt cells.

ABSTRACT: The intestinal epithelium is highly regenerative. Rapidly proliferating LGR5⁺ crypt base columnar (CBC) cells are responsible for epithelial turnover needed to maintain intestinal homeostasis. Upon tissue damage, loss of LGR5⁺ CBCs can be compensated by activation of quiescent +4 intestinal stem cells (ISCs) or early progenitor cells to restore intestinal regeneration. LGR5⁺ CBC self-renewal and ISC conversion to LGR5⁺ cells are regulated by external signals originating from the ISC niche. In contrast, little is known about intrinsic regulatory mechanisms critical for maintenance of LGR5⁺ CBC homeostasis. We found that LGR5 expression in intestinal crypt cells is controlled by the circadian core clock gene BMAL1 and the BMAL1-regulated

SUBMITTER: Cheng LT 

PROVIDER: S-EPMC10579233 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature