Nitrogen fixation and diazotroph diversity in groundwater systems.

ABSTRACT: Biological nitrogen fixation (BNF), the conversion of N₂ into bioavailable nitrogen (N), is the main process for replenishing N loss in the biosphere. However, BNF in groundwater systems remains poorly understood. In this study, we examined the activity, abundance, and community composition of diazotrophs in groundwater in the Hetao Plain of Inner Mongolia using ¹⁵N tracing methods, reverse transcription qPCR (RT-qPCR), and metagenomic/metatranscriptomic analyses. ¹⁵N₂ tracing incubation of near in situ groundwater (9.5-585.4 nmol N L^-1 h^-1) and N₂-fixer enrichment and isolates (13.2-1728.4 nmol N g^-1 h^-1, as directly verified by single-cell resonance Raman spectroscopy), suggested that BNF i

SUBMITTER: Liu X 

PROVIDER: S-EPMC10579273 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature