A cytoplasmic chemoreceptor and reactive oxygen species mediate bacterial chemotaxis to copper.

ABSTRACT: Chemotaxis is a widespread strategy used by unicellular and multicellular living organisms to maintain their fitness in stressful environments. We previously showed that bacteria can trigger a negative chemotactic response to a copper (Cu)-rich environment. Cu ion toxicity on bacterial cell physiology has been mainly linked to mismetallation events and reactive oxygen species (ROS) production, although the precise role of Cu-generated ROS remains largely debated. Here, using inductively coupled plasma optical emission spectrometry on cell fractionates, we found that the cytoplasmic Cu ion content mirrors variations of the extracellular Cu ion concentration. ROS-sensitive fluorescent probe and biosensor allowed us to show that the increase of cytoplasmic Cu ion content triggers a dose-depen

SUBMITTER: Louis G 

PROVIDER: S-EPMC10579534 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature