Preanalytical conditions for multiparameter platelet flow cytometry.

ABSTRACT:

Background

Flow cytometry is an important technique for understanding multiple aspects of blood platelet biology. Despite the widespread use of the platform for assessing platelet function, the optimization and careful consideration of preanalytical conditions, sample processing techniques, and data analysis strategies should be regularly assessed. When set up and designed with optimal conditions, it can ensure the acquisition of robust and reproducible flow cytometry data. However, these parameters are rarely described despite their importance.

Objectives

We aimed to characterize the effects of several preanalytical variables on the analysis of blood platelets by multiparameter fluorescent flow cytometry.

Methods

We assessed anticoagulant choice, sample material, sam

SUBMITTER: Hindle MS 

PROVIDER: S-EPMC10579537 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature