MALDI Mass Spectrometry Imaging and Semi-Quantification of Topically Delivered Lactic Acid.

ABSTRACT:

Background

Lactic acid is a common active ingredient in many topical skincare products; however, measuring its delivery into the skin is challenging due to the presence of a large level of endogenous lactic acid. In this study, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was used to quantitatively and qualitatively measure the delivery of lactic acid into the skin from a range of topical skincare products.

Materials and methods

Porcine skin samples were treated with various skincare products containing lactic acid. After 24 h, skin samples were sectioned and treated via H&E staining or prepared for MALDI-MSI using chemical derivatization. Samples were then analyzed by MALDI-MSI imaging to obtain lactic acid distribution in the entire ski

SUBMITTER: Cohen A 

PROVIDER: S-EPMC10579629 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature