Identifying potential biomarkers for non-obstructive azoospermia using WGCNA and machine learning algorithms.

ABSTRACT:

Objective

The cause and mechanism of non-obstructive azoospermia (NOA) is complicated; therefore, an effective therapy strategy is yet to be developed. This study aimed to analyse the pathogenesis of NOA at the molecular biological level and to identify the core regulatory genes, which could be utilised as potential biomarkers.

Methods

Three NOA microarray datasets (GSE45885, GSE108886, and GSE145467) were collected from the GEO database and merged into training sets; a further dataset (GSE45887) was then defined as the validation set. Differential gene analysis, consensus cluster analysis, and WGCNA were used to identify preliminary signature genes; then, enrichment analysis was applied to these previously screened signature genes. Next, 4 machine learning algorithms (RF, S

SUBMITTER: Tang Q 

PROVIDER: S-EPMC10579891 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature