Single-cell RNA sequencing analysis reveals transcriptional heterogeneity of multiple primary lung cancer.

ABSTRACT:

Introduction

With the advancements in early diagnosis, more and more patients with multiple primary lung cancer (MPLC) have been identified. However, the progression of MPLC involves complex changes in cell composition and metabolic function, which remains largely controversial.

Objective

Our study aims to comprehensively reveal the cellular characteristics and inter-cellular connections of MPLC.

Methods

We performed scRNA-seq from 23 samples of six MPLC patients, combined with bulk whole-exome sequencing. We performed trajectory analysis to investigate the transition of different cell types during the development of MPLC.

Results

A total of 1 67 397 cells were sequenced derived from tumour and adjacent tissues of MPLC patients, and tumour, normal, immune and s

SUBMITTER: Guo W 

PROVIDER: S-EPMC10580343 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature