Pregnane X receptor (PXR) deficiency protects against spinal cord injury by activating NRF2/HO-1 pathway.

ABSTRACT:

Introduction

As a devastating neurological disease, spinal cord injury (SCI) results in severe tissue loss and neurological dysfunction. Pregnane X receptor (PXR) is a ligand-activated nuclear receptor with a major regulatory role in xenobiotic and endobiotic metabolism and recently has been implicated in the central nervous system. In the present study, we aimed to investigate the role and mechanism of PXR in SCI.

Methods

The clip-compressive SCI model was performed in male wild-type C57BL/6 (PXR^+/+ ) and PXR-knockout (PXR^-/- ) mice. The N2a H₂ O₂ -induced injury model mimicked the pathological process of SCI in vitro. Pregnenolone 16α-carbonitrile (PCN), a mouse-specific PXR agonist, was used to activate PXR in vivo and in vitro.

SUBMITTER: Xuan LN 

PROVIDER: S-EPMC10580351 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature