Optimization of ultrasound-assisted extraction based on response surface methodology using HPLC-DAD for the analysis of red clover (Trifolium pretense L.) isoflavones and its anti-inflammatory activities on LPS-induced 3D4/2 cell.

ABSTRACT:

Introduction

Trifolium pratense L. has anti-inflammatory, antioxidant, cardiovascular disease prevention, and estrogen-like effects. The existing method for the assay of effective components is commonly based on a spectrophotometer, which could not meet the requirement of quality control. Furthermore, although there have been many studies on the anti-inflammation effect of red clover, a few have been reported on the regulatory effect of red clover isoflavones (RCI) on lipopolysaccharide (LPS)-induced inflammatory response in porcine alveolar macrophages (3D4/2 cells), and its mechanism of action is still unclear.

Methods

The main components of RCI including daidzein, genistein, and biochanin A were accurately quantified by high-performance liquid chromatography couple

SUBMITTER: Luo Z 

PROVIDER: S-EPMC10580807 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature