Approaches for timeline reductions in pathogenesis studies using genetically modified mice.

ABSTRACT: Although genetically modified mouse models have long been a powerful tool for microbiology research, the manipulation of the mouse genome is expensive, time consuming, and has historically remained the domain of dedicated animal facilities. The recent use of in vivo clustered regularly interspaced short palindromic repeats (CRISPR)-based editing technology has been reported to reduce the expertise, cost, and time required to generate novel mouse lines; it has remained unclear, however, if this new technology could meaningfully alter experimental timelines. Here, we report the optimization of an in oviduct murine genetic manipulation technique for use by microbiologists. We use this approach to generate a series of knockout mice and detail a protocol using an influenza A virus infect

SUBMITTER: Skavicus S 

PROVIDER: S-EPMC10580824 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature