Transposon sequencing identifies genes impacting Staphylococcus aureus invasion in a human macrophage model.

ABSTRACT: Staphylococcus aureus is a facultative intracellular pathogen in many host cell types, facilitating its persistence in chronic infections. The genes contributing to intracellular pathogenesis have not yet been fully enumerated. Here, we cataloged genes influencing S. aureus invasion and survival within human THP-1 derived macrophages using two laboratory strains (ATCC2913 and JE2). We developed an in vitro transposition method to produce highly saturated transposon mutant libraries in S. aureus and performed transposon insertion sequencing (Tn-Seq) to identify candidate genes with significantly altered abundance following macrophage invasion. While some significant genes were strain-specific, 108 were identified as common across both S. aureus strains, wi

SUBMITTER: Lo H-Y 

PROVIDER: S-EPMC10580828 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature