Detrimental impact of the Geobacter metallireducens type VI secretion system on direct interspecies electron transfer.

ABSTRACT: Direct interspecies electron transfer (DIET) is important in anaerobic communities of environmental and practical significance. Other than the need for close physical contact for electrical connections, the interactions of DIET partners are poorly understood. Type VI secretion systems (T6SSs) typically kill competitive microbes. Surprisingly, Geobacter metallireducens highly expressed T6SS genes when DIET-based co-cultures were initiated with Geobacter sulfurreducens. T6SS gene expression was lower when the electron shuttle anthraquinone-2,6-disulfonate was added to alleviate the need for interspecies contact. Disruption of hcp, the G. metallireducens gene for the main T6SS needle-tube protein subunit, and the most highly upregulated gene in DIET-grown cells eli

SUBMITTER: Smith JA 

PROVIDER: S-EPMC10580878 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature