Novel mechanism of hydrogen peroxide for promoting efficient natamycin synthesis in Streptomyces.

ABSTRACT: The mechanism of regulation of natamycin biosynthesis by Streptomyces in response to oxidative stress is unclear. Here, we first show cholesterol oxidase SgnE, which catalyzes the formation of H₂O₂ from sterols, triggered a series of redox-dependent interactions to stimulate natamycin production in S. gilvosporeus. In response to reactive oxygen species, residues Cys212 and Cys221 of the H₂O₂-sensing consensus sequence of OxyR were oxidized, resulting in conformational changes in the protein: OxyR extended its DNA-binding domain to interact with four motifs of promoter p _sgnM . This acted as a redox-dependent switch to turn on/off gene transcription of sgnM, which encodes a cluster-situated regulator, by controllin

SUBMITTER: Zong G 

PROVIDER: S-EPMC10580950 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature