Transcriptomic and genomic effects of gamma-radiation exposure on strains of the black yeast Exophiala dermatitidis evolved to display increased ionizing radiation resistance.

ABSTRACT: Melanized fungi thrive in extreme environments, including those with high levels of ionizing radiation. To understand the role that melanin may play in ionizing radiation resistance, we previously performed an adaptive laboratory evolution experiment in which we used melanized and non-melanized strains of the yeast Exophiala dermatitidis to develop evolved lines that exhibit increased ionizing radiation resistance. In this study, we further characterized these evolved lines by analyzing their response to ionizing radiation at the transcriptomic and genomic levels. RNA sequencing showed that the response to gamma irradiation in both unevolved and evolved strains involved the induction of DNA repair genes. However, in the melanized lines evolved to exhibit increased ionizing radiation

SUBMITTER: Yuzon JD 

PROVIDER: S-EPMC10581076 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature