Regulatory response to a hybrid ancestral nitrogenase in Azotobacter vinelandii.

ABSTRACT: Biological nitrogen fixation, the microbial reduction of atmospheric nitrogen to bioavailable ammonia, represents both a major limitation on biological productivity and a highly desirable engineering target for synthetic biology. However, the engineering of nitrogen fixation requires an integrated understanding of how the gene regulatory dynamics of host diazotrophs respond across sequence-function space of its central catalytic metalloenzyme, nitrogenase. Here, we interrogate this relationship by analyzing the transcriptome of Azotobacter vinelandii engineered with a phylogenetically inferred ancestral nitrogenase protein variant. The engineered strain exhibits reduced cellular nitrogenase activity but recovers wild-type growth rates following an extended lag period. We find that e

SUBMITTER: Rivier AJ 

PROVIDER: S-EPMC10581106 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature