GraS signaling in Staphylococcus aureus is regulated by a single D35 residue in the extracellular loop.

ABSTRACT: Bacterial two-component systems are crucial features of bacterial pathogens such as methicillin-resistant Staphylococcus aureus to overcome environmental and antimicrobial stresses by activating regulons to interfere with the bactericidal mechanisms. GraRS is a unique subset of two-component systems belonging to the intramembrane-sensing histidine kinase family (IM-HK) and is responsible for resistance to cationic host defense peptides. However, the precise manner by which the short 9-residue extracellular loop of the membrane sensor GraS detects the antimicrobial peptides and transduces the signal is not comprehensively understood. Here, we show that a single point mutation (D35A) in the extracellular loop of GraS blocked activation of GraRS, but this effect was also abrogated with

SUBMITTER: Cho J 

PROVIDER: S-EPMC10581149 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature