CRISPR-based tools for targeted genetic manipulation in pathogenic Sporothrix species.

ABSTRACT: Sporothrix brasiliensis is an emerging fungal pathogen frequently associated with zoonotic transmission of sporotrichosis by contaminated cats. Within 25 years, the disease has spread not only throughout Brazil but now to neighboring countries in Latin America. Thermo-dimorphism, melanin, glycans, adhesins, and secreted vesicles have been associated with the ability of Sporothrix species to cause disease in the mammalian host. Although certain virulence factors have been proposed as potential determinants for sporotrichosis, the scarcity of molecular tools for performing reverse genetics in Sporothrix has significantly impeded the dissection of mechanisms underlying the disease. Here, we demonstrate that PEG-mediated protoplast transformation is a powerful method for h

SUBMITTER: Hatinguais R 

PROVIDER: S-EPMC10581184 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature