Targeted capillary photothrombosis via multiphoton excitation of Rose Bengal.

ABSTRACT: Microvascular stalling, the process occurring when a capillary temporarily loses perfusion, has gained increasing interest in recent years through its demonstrated presence in various neuropathologies. Studying the impact of such stalls on the surrounding brain tissue is of paramount importance to understand their role in such diseases. Despite efforts trying to study the stalling events, investigations are hampered by their elusiveness and scarcity. In an attempt to alleviate these hurdles, we present here a novel methodology enabling transient occlusions of targeted microvascular segments through multiphoton excitation of Rose Bengal, an established photothrombotic agent. With n = 7 mice C57BL/6 J (5 males and 2 females) and 95 photothrombosis trials, we demonstrate the ability of trigge

SUBMITTER: Delafontaine-Martel P 

PROVIDER: S-EPMC10581236 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature