Screening in serum-derived medium reveals differential response to compounds targeting metabolism.

ABSTRACT: A challenge for screening new anticancer drugs is that efficacy in cell culture models is not always predictive of efficacy in patients. One limitation of standard cell culture is a reliance on non-physiological nutrient levels, which can influence cell metabolism and drug sensitivity. A general assessment of how physiological nutrients affect cancer cell response to small molecule therapies is lacking. To address this, we developed a serum-derived culture medium that supports the proliferation of diverse cancer cell lines and is amenable to high-throughput screening. We screened several small molecule libraries and found that compounds targeting metabolic enzymes were differentially effective in standard compared to serum-derived medium. We exploited the differences in nutrient levels bet

SUBMITTER: Abbott KL 

PROVIDER: S-EPMC10581593 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature