Endothelial-derived von Willebrand factor accelerates fibrin clotting within engineered microvessels.

ABSTRACT:

Background

Von Willebrand factor (VWF) is classically associated with primary hemostasis and platelet-rich arterial thromboses, but recently has also been implicated in fibrin clotting and venous thrombosis. Direct interaction between fibrin and VWF may mediate these processes, although prior reports are conflicting.

Objectives

We combined two complementary platforms to characterize VWF-fibrin(ogen) interactions and identify their potential physiologic significance.

Methods

Engineered microvessels were lined with human endothelial cells, cultured under flow, and activated to release VWF and form transluminal VWF fibers. Fibrinogen, fibrin monomers, or polymerizing fibrin were then perfused, and interactions with VWF evaluated. Thrombin and fibrinogen were perfused int

SUBMITTER: Rayner SG 

PROVIDER: S-EPMC10581744 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature