Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD.

ABSTRACT: In recent years, meat adulteration safety incidents have occurred frequently, triggering widespread attention and discussion. Although there are a variety of meat quality identification methods, conventional assays require high standards for personnel and experimental conditions and are not suitable for on-site testing. Therefore, there is an urgent need for a rapid, sensitive, high specificity and high sensitivity on-site meat detection method. This study is the first to apply RPA combined with CRISPR/Cas12a technology to the field of multiple meat identification. The system developed by parameter optimization can achieve specific detection of chicken, duck, beef, pork and lamb with a minimum target sequence copy number as low as 1 × 10⁰ copies/μL for 60 min at a constant tempe

SUBMITTER: Liu Y 

PROVIDER: S-EPMC10582345 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature