Improving LC-MS/MS measurements of steroids with differential mobility spectrometry.

ABSTRACT:

Introduction

Steroid measurements are important for diagnosis and monitoring of many conditions and treatment regiments; however, due to structural and chemical similarities amongst steroids, these analyses are challenging, even for highly specific techniques such as liquid chromatography-tandem mass spectrometry (LC-MS/MS). Differential mobility spectrometry (DMS) has the potential to improve these analyses by providing an orthogonal and complementary separation technique.

Methods

Initially, the potential for DMS to improve signal-to-noise ratio (S/N) and reduce interference was tested by comparing chromatograms acquired with and without DMS when performing measurements of six different steroids. Subsequently, a full clinical validation of cortisol and cortisone in urine wa

SUBMITTER: Chai Y 

PROVIDER: S-EPMC10582739 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature