Intestinal microbiota modulation at the strain level by the olive oil polyphenols in the diet.

ABSTRACT:

Introduction

Previously we have reported a r16S gene next generation sequencing study on the effect of high fat diets in the intestinal microbiota using a murine model. However, many important microbial traits occur at strain level and, in order to detect these population changes, culture-dependent approaches need to be applied. With this goal, we decided to study a very well-known commensal genus, Enterococcus, and therefore, intestinal enterococci methodically isolated during the above-mentioned experiment were analyzed.

Materials and methods

A collection of 75 distinct enterococcal strains isolated from feces of mice fed a standard diet or high-fat diets enriched with butter, refined olive oil, or extra virgin olive oil and after 0, 6 or 12 weeks of diet, were gene

SUBMITTER: Andujar-Tenorio N 

PROVIDER: S-EPMC10582982 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature