Time-domain proton-detected local-field NMR for molecular structure determination in complex lipid membranes.

ABSTRACT: Proton-detected local-field (PDLF) NMR spectroscopy, using magic-angle spinning and dipolar recoupling, is presently the most powerful experimental technique for obtaining atomistic structural information from small molecules undergoing anisotropic motion. Common examples include peptides, drugs, or lipids in model membranes and molecules that form liquid crystals. The measurements on complex systems are however compromised by the larger number of transients required. Retaining sufficient spectral quality in the direct dimension requires that the indirect time-domain modulation becomes too short for yielding dipolar splittings in the frequency domain. In such cases, the dipolar couplings can be obtained by fitting the experimental data; however ideal models often fail to fit PDLF data prop

SUBMITTER: Wurl A 

PROVIDER: S-EPMC10583295 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature