Identification and characterization of differentially expressed circular RNAs in extraocular muscle of oculomotor nerve palsy.

ABSTRACT:

Background

Oculomotor nerve palsy (ONP) is a neuroparalytic disorder resulting in dysfunction of innervating extraocular muscles (EOMs), of which the pathological characteristics remain underexplored.

Methods

In this study, medial rectus muscle tissue samples from four ONP patients and four constant exotropia (CXT) patients were collected for RNA sequencing. Differentially expressed circular RNAs (circRNAs) were identified and included in functional enrichment analysis, followed by interaction analysis with microRNAs and mRNAs as well as RNA binding proteins. Furthermore, RT-qPCR was used to validate the expression level of the differentially expressed circRNAs.

Results

A total of 84 differentially expressed circRNAs were identified from 10,504 predicted circRNAs. Fun

SUBMITTER: Shi M 

PROVIDER: S-EPMC10583365 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature