AAV-compatible optogenetic tools for activating endogenous calcium channels in vivo.

ABSTRACT: Calcium ions (Ca²⁺) play pivotal roles in regulating diverse brain functions, including cognition, emotion, locomotion, and learning and memory. These functions are intricately regulated by a variety of Ca²⁺-dependent cellular processes, encompassing synaptic plasticity, neuro/gliotransmitter release, and gene expression. In our previous work, we developed 'monster OptoSTIM1' (monSTIM1), an improved OptoSTIM1 that selectively activates Ca²⁺-release-activated Ca²⁺ (CRAC) channels in the plasma membrane through blue light, allowing precise control over intracellular Ca²⁺ signaling and specific brain functions. However, the large size of the coding sequence of monSTIM1 poses a limitation for its widespread use, as it exceeds the packaging

SUBMITTER: Kook YH 

PROVIDER: S-EPMC10583393 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature