Regulation of secondary hair follicle cycle in cashmere goats by miR-877-3p targeting IGFBP5 gene.

ABSTRACT: Cashmere, a highly valuable animal product derived from cashmere goats, holds significant economic importance. MiRNAs serve as crucial regulators in the developmental processes of mammalian hair follicles. Understanding the regulation of miRNAs during the hair follicle cycle is essential for enhancing cashmere quality. In this investigation, we employed high-throughput sequencing technology to analyze the expression profiles of miRNAs in the secondary hair follicles of Jiangnan cashmere goats at different stages. Through bioinformatics analysis, we identified differentially expressed miRNAs (DE miRNAs). The regulatory relationships between miRNAs and their target genes were verified using multiple techniques, including RT-qPCR, western blot, Dual-Luciferase Reporter, and CKK-8 assays. Our

SUBMITTER: Wu C 

PROVIDER: S-EPMC10583983 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature