Combined effect of heat and corona charge on molecular delivery to a T cell line in vitro.

ABSTRACT: With the rapid increase of gene and immunotherapies for treating cancer, there is a need to efficiently transfect cells. Previous studies suggest that electrotransfer can provide a non-viral method for gene delivery. Electrotransfer traditionally relies upon the application of direct current pulses to the cells of interest. Corona charge was investigated in this study as an alternative to traditional methods as a means of creating the electric field necessary to deliver materials via electrotransfer. The goal was to determine if there was an increase in molecular delivery across the membrane of a human T cell line used as a model system. In a novel dish created for the study, the effects of elevated temperatures (37, 40, 43, and 45°C) during the treatment process were also examined in comb

SUBMITTER: Skinner MA 

PROVIDER: S-EPMC10584139 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature