A pentameric TRPV3 channel with a dilated pore.

ABSTRACT: Transient receptor potential (TRP) channels are a large, eukaryotic ion channel superfamily that control diverse physiological functions, and therefore are attractive drug targets^1-5. More than 210 structures from more than 20 different TRP channels have been determined, and all are tetramers⁴. Despite this wealth of structures, many aspects concerning TRPV channels remain poorly understood, including the pore-dilation phenomenon, whereby prolonged activation leads to increased conductance, permeability to large ions and loss of rectification^6,7. Here, we used high-speed atomic force microscopy (HS-AFM) to analyse membrane-embedded TRPV3 at the single-molecule level and discovered a pentameric state. HS-AFM dynamic imaging revealed transience and reversibil

SUBMITTER: Lansky S 

PROVIDER: S-EPMC10584365 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature