Ascorbic acid enhances the cold preservation period of human adipose tissue-derived mesenchymal stromal cells.

ABSTRACT:

Introduction

We previously developed 3% trehalose-added lactated Ringer's solution (LR-3T) and 3% trehalose- and 5% dextran-40-added lactated Ringer's solution (LR-3T-5D), which can be used to preserve adipose-derived mesenchymal stem cells (hADSCs) for 24 h at 5 and 25 °C. However, it is necessary to further extend the storage duration of cells to expand transportation zones and ensure time for quality control testing of final cell products. Therefore, we attempted to prolong the preservation duration of hADSCs by adding supplements to LR-3T-5D. We focused on ascorbic acid as an antioxidant because it is widely clinically as a nutrient.

Methods

We added the antioxidant ascorbic acid to LR-3T-5D and evaluated the viability, colony formation rate, proliferative capacity, and

SUBMITTER: Wada T 

PROVIDER: S-EPMC10584694 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature