Human thymic putative CD8αα precursors exhibit a biased TCR repertoire in single cell AIRR-seq.

ABSTRACT: Thymic T cell development comprises T cell receptor (TCR) recombination and assessment of TCR avidity towards self-peptide-MHC complexes presented by antigen-presenting cells. Self-reactivity may lead to negative selection, or to agonist selection and differentiation into unconventional lineages such as regulatory T cells and CD8[Formula: see text] T cells. To explore the effect of the adaptive immune receptor repertoire on thymocyte developmental decisions, we performed single cell adaptive immune receptor repertoire sequencing (scAIRR-seq) of thymocytes from human young paediatric thymi and blood. Thymic PDCD1⁺ cells, a putative CD8[Formula: see text] T cell precursor population, exhibited several TCR features previously associated with thymic and peripheral ZNF683⁺

SUBMITTER: Heimli M 

PROVIDER: S-EPMC10584817 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature