Bioplastic (poly-3-hydroxybutyrate)-producing Massilia endophytica sp. nov., isolated from Cannabis sativa L. 'Cheungsam'.

ABSTRACT: A rod-shaped, motile, Gram-negative bacterial strain named DM-R-R2A-13^T was isolated from the plant Cannabis sativa L. 'Cheungsam'. The phylogenetic analysis of the 16S rRNA gene sequence revealed that strain DM-R-R2A-13^T belongs to the family Oxalobacteraceae and is closely related to members of the genus Massilia, with Massilia flava (97.58% sequence similarity) and Massilia armeniaca (97.37% sequence similarity) being the closest members. The digital DNA-DNA hybridization (dDDH) values between strain DM-R-R2A-13^T and Massilia flava CGMCC 1.10685^T and Massilia armeniaca ZMN-3^Twere 22.2% and 23.3%, while the average nucleotide identity (ANI) values were 78.85% and 79.63%, respectively. The DNA G+C content was measured to be 64.6 mol%.

SUBMITTER: Jeon D 

PROVIDER: S-EPMC10584911 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature