Single cell RNA-sequencing analysis reveals that N-acetylcysteine partially reverses hepatic immune dysfunction in biliary atresia.

ABSTRACT:

Background & aims

Our previous study indicated that CD177⁺ neutrophil activation has a vital role in the pathogenesis of biliary atresia (BA), which is partially ameliorated by N-acetylcysteine (NAC) treatment. Here, we evaluated the clinical efficacy of NAC treatment and profiled liver-resident immune cells via single cell RNA-sequencing (scRNA-seq) analysis to provide a comprehensive immune landscape of NAC-derived immune regulation.

Methods

A pilot clinical study was conducted to evaluate the potential effects of intravenous NAC treatment on infants with BA, and a 3-month follow-up was carried out to assess treatment efficacy. scRNA-seq analysis of liver CD45⁺ immune cells in the control (n = 4), BA (n = 6), and BA + NAC (n = 6) groups was per

SUBMITTER: Ye R 

PROVIDER: S-EPMC10585304 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature