Particle profiling of EV-lipoprotein mixtures by AFM nanomechanical imaging.

ABSTRACT: The widely overlapping physicochemical properties of lipoproteins (LPs) and extracellular vesicles (EVs) represents one of the main obstacles for the isolation and characterization of these pervasive biogenic lipid nanoparticles. We herein present the application of an atomic force microscopy (AFM)-based quantitative morphometry assay to the rapid nanomechanical screening of mixed LPs and EVs samples. The method can determine the diameter and the mechanical stiffness of hundreds of individual nanometric objects within few hours. The obtained diameters are in quantitative accord with those measured via cryo-electron microscopy (cryo-EM); the assignment of specific nanomechanical readout to each object enables the simultaneous discrimination of co-isolated EVs and LPs even if they have overl

SUBMITTER: Ridolfi A 

PROVIDER: S-EPMC10585431 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature